anti cd8  (Bio-Rad)

Journal: NPJ Parkinson's Disease

Article Title: Delphinidin modulates neuroinflammation and behavioral deficits in a Parkinson’s disease mouse model

doi: 10.1038/s41531-025-01244-0

Figure Lengend Snippet: a Quantification of CD4 + cells in the SN. Statistical analysis by one-way ANOVA followed by Tukey’s multiple comparisons test: F(3, 12) = 2.156, P = 0.1464, n-numbers: EV veh = 3, EV del = 4, hαSYN veh = 5, hαSYN del = 4 b Quantification of CD8 + cells in the substantia nigra (SN). Statistical analysis by one-way ANOVA followed by Tukey’s multiple comparisons test: F(3, 12) = 5.524, P = 0.0129, n-numbers: EV veh = 3, EV del = 4, hαSYN veh = 5, hαSYN del = 4 c Quantification of CD4 + cells in the striatum. Statistical analysis by one-way ANOVA followed by Tukey’s multiple comparisons test: F(3, 11) = 1.215, P = 0.3501, n-numbers: EV veh = 3, EV del = 4, hαSYN veh = 4, hαSYN del = 4 d Quantification of CD8 + cells in the striatum. Statistical analysis by one-way ANOVA followed by Tukey’s multiple comparisons test: F(3, 11) = 0.2133, P = 0.8851, n-numbers: EV veh = 3, EV del = 4, hαSYN veh = 4, hαSYN del = 4 e Representative IF images of CD8 + CD122 + cells in the SN. Scale bar: 50 µm f Quantification of CD8 + CD122 + cells in the SN – as indicated by TH + cells. Statistical analysis by one-tailed multiple t-tests, only showing significant results, P = 0.0425 (hαSYN veh vs. hαSYN del ), P = 0.0270 (EV veh vs. hαSYN del ), n-numbers: EV veh = 6, EV del = 6, hαSYN veh = 6, hαSYN del = 6; *P <0.05, **P <0.01, ***P <0.001, ****P <0.0001. Data are shown as min-to-max and mean (+).

Article Snippet: Subsequently, sections were incubated overnight with rat anti-mouse CD4 (1:1000, Bio-Rad, cat. #MCA1767; RRID:AB_322769), rat anti-mouse CD8 (1:500, Bio-Rad, cat. #MCA609G; RRID:AB_321407), rat anti-mouse CD11b (1:100, Bio-Rad, cat. #MCA711; RRID:AB_321292), or rabbit anti-TH antibodies.

Techniques: One-tailed Test

Journal: Animal Cells and Systems

Article Title: Novel TLR2 agonist Amuc_C derived from Akkermansia muciniphila exhibits potent anti-tumor activity in colorectal cancers

doi: 10.1080/19768354.2025.2578019

Figure Lengend Snippet: The immunofluorescence staining of CT26 tumors harvested from control and Amuc_C-treated groups. (A) T cell panel. CD3, CD8, and CD4 were stained in green, red, and pink; (B) M1 macrophage panel. CD86 and iNOS were stained pink and red; (C) M2 macrophage panel. CD206 and arginase-1 (Arg-1) were stained in red and pink. Cell nuclei were stained with DAPI. (scale bar = 20 μm). Images were quantified randomly in five fields at 20x magnification. All data are presented as mean ± SEM. Statistical significance was calculated by one-way ANOVA with the Tukey test for the comparisons between all groups (ns, not significant; *, p < 0.05; ****, p < 0.0001).

Article Snippet: The following monoclonal antibodies were used to assess the phenotypes of T lymphocytes, macrophages, and dendritic cells: PE/Cyanine7 anti-mouse CD45 (BioLegend, clone 30-F11, Cat. No. 103114), FITC anti-mouse Ly-6C (BioLegend, clone HK1.4, Cat. No. 128006), rat anti-Mouse CD3: FITC (Bio–Rad, Hercules, CA, USA, clone KT3, Cat. No. MCA500F), rat anti-Mouse CD4: RPE (Bio–Rad, clone RM4-5, Cat. No. MCA2691PE), rat anti-Mouse CD8: Alexa Fluor® 647 (Bio–-Rad, clone YTS169.4, Cat. No. MCA1768A647), PE anti-mouse F4/80 (BioLegend, clone BM8, Cat. No. 123110), APC/Cyanine7 anti-mouse/human CD11b (BioLegend, clone M1/70, Cat. No. 101226), PerCP/Cyanine5.5 anti-mouse I-A/I-E (BioLegend, clone M5/114.15.2, Cat. No. 107626), APC anti-mouse CD11c (BioLegend, clone N418, Cat. No. 117310), and PE anti-mouse CD103 (BioLegend, clone 2E7, Cat. No. 121406).

Techniques: Immunofluorescence, Staining, Control